Ensure that the required contact time for the sanitizer has been achieved. Water column sampling using dedicated sampler devices specially designed to assess the impact of offshore activities on sea water quality Groundwater sampling Collection of groundwater and analysis to international standards using a comprehensive range of equipment, including interface meters, low and high flow sampling pumps, flow through cells and calibrated field instrumentation To lay down the procedure for Sampling and Testing of Purified Water. If the Total bacterial count exceeds the Alert limit/Action limit, immediately inform the same to QA and Engineering personnel and investigation shall be done as per the SOP for “Handling of microbial Excursion in Water and Environment”. Standard Operating Procedure (SOP) for sampling from different points and microbiological analysis/testing of Purified water samples in pharmaceutical plants. endobj If sampling from a water faucet, run the faucet for 1 minute before obtaining a sample. Purified water quality parameter trending shall be done on the Monthly basis, trend charts shall be reviewed and a conclusion shall be made (if required). Alert levels are always lower than Action levels. If a hose is already connected to the port or valve, collect the sample directly from that hose. It represents one aspect of water quality. for validated time. Wear gloves and nose mask before sampling. – How can we meet the demands microbiological water AQC in the future? Related: SOP for Corrective and Preventive Action (CAPA), List of SOP for Pharmaceutical Quality Assurance, SOP on Handling of Incidents and Deviations, SOP ON Receipt, Issue & Control Of Lab Chemicals, SOP for Qualification of Quality Control analyst, SOP On Safety In Quality Control Laboratory, SOP for Corrective and Preventive Action (CAPA), ← SOP For Handling of Power Failure in Plant, List of ICH Quality Guidelines for Pharmaceutical Industry, SOP List For Pharmaceutical Quality Assurance, Chemical TOC /Oxidisable Substance Total Microbial Counts Presence of Pathogens, Total microbial counts Presence of Pathogens. Then remove the sterile strip from the bottle. 2 0 obj Carry the sampling containers to the sampling point as per the sampling schedule. • Sampling points should be located in such a way that water can be sampled from reserve tanks and reservoirs, etc. If no sterile bottle is available, rinse the bottle first with hot water, then with the sample and then proceed to fill the bottle with sample. If you would like the entire volume, please send your email request for a zip file.. Officer or Executive of the Microbiology department shall be responsible for the preparation of new or revision of existing SOP. Open the valve of the sampling point and allow the water to drain for about 20-30 seconds. A designated person shall issue the template/worksheet. Fill the bottle almost to the top, leaving a gap for mixing (approx 5 mm). Faecal contamination of water is routinely detected by microbiological analysis. PROCEDURE FOR MICROBIOLOGICAL MONITORING OF WATER. Carry out the chemical analysis of samples as per the current version of purified water specification. Sampling water for subsequent analysis is often considered to be somewhat easier than sampling, say, soils for two main reasons: (i) water tends to be more homogeneous than soils, there is less point-to-point variability between two samples collected within the same vicinity, (ii) it is often physically easier to collect water samples because it can be done with pumps and hose lines, (iii) known volumes of water can … Water system sanitization methods require assessment. OBJECTIVE:To lay down a procedure for microbiological monitoring of raw water and purified water. Wear gloves when collecting samples. ACoP L8 recommends that sampling for Legionella should be carried out when: In particular, the spread of enteric viruses, e.g. 2 Content • Introduction – What does the future hold for microbiological water AQC? Collect your water sample early in the week – Monday to Thursday –to give enough time to get to the laboratory and to be analyzed within 24-30 hours. <> 3 0 obj W|b1*]�y.����>'O�ak�Oߜ�~8� ���9X�;�����#|������O�en�4�O# Water Sampling Procedure: 1. Collection of purified water for Microbiological analysis: Hold the sterile container near the sampling point. Analyze the TOC sample as quickly as possible on arrival at the laboratory. Noroviruses, are repeatedly related to poor water quality (… 4.11 Open the valve of sampling point slowly to full and drain water for approx. Head of the Department / Designee of respective areas & QA shall be responsible for reviewing the SOPs. Flush water through sampling point for 5 – 20 Seconds. In water systems with inadequate quality control and sanitation, water could act as a vehicle for pathogenic microorganisms that originate from the faeces of wildlife including birds, livestock and pet animals, as well as humans. Carefully replace the cap. Sampling personnel shall wear clean smocks or gowns and sterile gloves prior to collecting any sample (As per area requirements). It is a microbiological analytical procedure which uses samples of water and from these samples determines the concentration of bacteria. Analyze the sample of purified water for pathogens using filtration technique/Direct as per the current specification of Purified water. 4 0 obj Sampling apparatus and containers shall be clean, sterile, and/or endotoxin-free as per test requirements. It is important to routinely identify organisms recovered from water systems when: Waterborne organisms may be detrimental to finished products or processes in which the water is used. endobj It is impractical to attempt the routine isolation of pathogens because they are present in relatively small numbers compared with other types of micro-organism. 1 minute. • In systems with more than one water source, the locations of the sampling points should take account of the number of inhabitants served by each source. Microbiological Analysis of Water Quality •Looking in to the 21st Century sigma-aldrich.com January 31, 2012 By Richard Glindon Presented by Klaus Herick. Step Three Remove Faucet Screen and 3. Correct sampling procedures as instructed by international standard protocols must be adhered to; Routine microbiological testing of drinking water supplies, recreational waters, and environmental waters is essential for the protection of public health ; Programme of testing. Close the container immediately and record the sampling time. Collect the 100ml of water sample for self analysis and 500ml of water for outside lab for microbiological analysis … 7`rV�XH���ق�*�r��θ �NMsp�^%B�+$��3�V�&�y�J09;��������;�[KeR)����XA_�ώ�z5]�Un6)���\��ٻ���O���T�E���\J0^5�4?���xE��� The changes may occur in the bacterial content of water on storage can be reduced to a minimum by ensuring that samples are not exposed to light and are kept cool. �d��5�ֹܵܯ�y!%r%��]iHg�3�g����U}�%�^u]}�l���Y{��������S}}����v��u���q�������""�trv���H2�O$F%�1�)���ϟe�5��ϟ�ϒ�o�ٿ�? Head QA shall be responsible for the approval of SOP. Wash 125 ml Amber glass or suitable bottles with a stopper with 2.0 M HCL or 2.0% nitric acid and rinse with purified water and dry the bottles at 105 ºC for at least one hour. Flush the sample port/valve for the qualified time or volume and immediately open the labeled sample container and collect the sample. Rinse the container at least one to two times with purified water from the sampling point. 5.2.1 Pour Plate Method Dispense one ml of sample into two Petri dishes. <> RESPONSIBILITY Quality Control Executive / Officer ACCOUNTABILITY Quality Assurance Head. 5.1.4 For a sampling of Treated Raw water (May contain chlorine), add 0.1 ml of 5 % sodium thiosulphate solution in the bottles before sterilization. Refer Annexure-2 for preparing the Sampling Schedule. <>/XObject<>/ProcSet[/PDF/Text/ImageB/ImageC/ImageI] >>/MediaBox[ 0 0 612 792] /Contents 4 0 R/Group<>/Tabs/S/StructParents 0>> The Laboratory Procedures for the Microbiological Analysis of Foods are available upon request. Talk to the lab to determine if this collection procedure is acceptable for the analytical method they plan to perform. If sampling a body of running water, point the mouth of the bag upstream and your hands downstream to avoid contamination. Rinse with purified water. Wrap the neck with aluminum foil to avoid any contamination during transportation. make the schedule in such manner that generation point (For Chemical & Microbiology Analysis) & return loop (For Microbiology Analysis only) covered on a daily basis and other useful points (For Microbiology Analysis only) to be covered in rotation within a month. <>>> The precautions taken to avoid contamination must be such that they do not affect any microorganisms that are to be revealed in the test (water analysis). Analyze the sample of purified water for Total Viable Count using filtration technique as per the current specification of Purified Water. surroundings dusty, covered with debris, or poorly ventilated; 2. Sanitize the hands with 70 % v/v IPA. Food Safety and Inspection Service: •Sampling methods •Assessing sampling plans and testing methods •Method validations and laboratory quality assurance •FSIS testing programs, methods and pathogen-specific issues to consider •Industry testing activities 2 Today’s Presentation. If the immediate analysis is not possible to acidify the sample with a 2-3 drop of 2.0 M HCL and store the sample under refrigeration at 2 to 8ºC. Annexure-1: List of Sampling Points of Purified Water. OBJECTIVE To lay down a procedure for microbiological monitoring of raw water and purified water SCOPE: This SOP shall be applicable for sampling and microbial analysis of raw and purified water from all user points. Collect the purified water sample (refer table 1) without rinsing and close it with stopper immediately. Close the valve and bring the samples to the laboratory and make an entry in the purified water inward register. Sampling … The sampling procedures and … Annexure-3: Sampling Details of Purified Water. Surface water sampling methods and analysis — technical appendices Standard operating procedures for water sampling- methods and analysis Looking after all our water needs Department of Water … 5.1.3 Samples for microbiological evaluation should be collected in Pre-sterilized screw cap glass bottles. 4. Chemical analysis: Note: Before sampling, the aluminum foil covering the sampling point should be removed. Clean 250 ml Clear glass bottles with cap for membrane filtration method or pour plate method and rinse with purified water. Sampling Procedures for Drinking Waters 1 PURPOSE This standard operating procedure outlines details for collection of drinking water samples for chemical, microbiological and radiological analysis. Analyze the sample as quickly as possible on arrival at the laboratory. MICROBIOLOGICAL WATER SAMPLING - BASIC PROCEDURES Taking a Routine Bacteria Sample Properly Step One Do Not Rinse The Bottle The powder in the bottle is meant to be there and will not contaminate your sample. endobj 1. WATER ANALYSIS SAMPLING PROCEDURES Microbiological samples: Microbiological samples should be collected in sterile plastic or glass bottles which Vinlab supplies. Unsatisfactory environmental condition e.g. Bacteriological water analysis is a method of analysing water to estimate the numbers of bacteria present and, if needed, to find out what sort of bacteria they are. For Microbiological Analysis: Clean 250 ml Clear glass bottles with cap for membrane filtration method or pour plate method and rinse with purified water. 4.2 Rinse water sampling: 4.2.1 After cleaning, the Production Officer shall inform to QA Officer for the collection of the rinse water sample. 4.2.2 Enter into the respective area and visually observe the machine or part of the machine. 6.0 PROCEDURE FOR ANALYSIS OF WATER SAMPLE: Carry out the determination under conditions designed to avoid extrinsic microbial contamination of the water sample for the analysis. Approximately add 15-20 ml of R2A / Plate count Agar into each Petri dishes. Domestic water systems are supplied with water fit to drink, so ACoP L8 advices that regular microbiological sampling is not required. 2. 3. If the following situation(s) is/are encountered, DO NOT TAKE SAMPLES. Prepare the sampling schedule (Tentative) by the first working day of every New Year. Phase 2 follows the determination of data validity and investigates the water system and all potentially affected lots of products. SUPPLY … There is a need to identify the source of contamination. SCOPE:This SOP shall provide the procedure for sampling and testing of raw water and purified water from all the user points and all the points across the critical functions in the water purification process. Ensure you have read the ‘Standard drinking water sampling procedure – microbiological’. Microbiologist shall wear sterilised gloves during the sampling activity. x��[mo�6� �Aw�X_DIE�q�;�:M � Replace the used hose onto the provided clamp after sampling so that it fully drains. These Alert levels can be modified depending on the trend analysis done in the monitoring program. Step Two Wash Your Hands prior to taking the sample. 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